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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Affinity purified, lyophilized
- 保存条件:
参照说明书
- 克隆性:
MEM-166
- 适应物种:
human
- 抗原来源:
Mouse
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 宿主:
Mouse
- 应用范围:
IHC
- 抗体英文名:
CD177 (Neutrophil-Specific Antigen NB1), mouse anti human, clone MEM-166
- 抗体名:
CD177 (Neutrophil-Specific Antigen NB1), mouse anti human, clone MEM-166
- 规格:
100µg
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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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