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- 2025年12月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
6个月
- 英文名：
Cellazyme C Tablets
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- 保存条件：
常温
- 规格：
200 Tablets/1000 Tablets
| 规格： | 200 Tablets | 产品价格： | ￥5967.0 |
|---|---|---|---|
| 规格： | 1000 Tablets | 产品价格： | ￥21838.0 |
High purity dyed and crosslinked Cellazyme C tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of endo-cellulase. Containing AZCL-HE-Cellulose. Recommended substrate for the assay of endo-cellulase.
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 6 months under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
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- 作者
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文献和实验1.Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter.
Inokuma, K., Hasunuma, T. & Kondo, A.(2014). Biotechnology for Biofuels, 7(1):8.
2.Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina.
Stricker, A., R., Steiger, M., G. & Mach, R., L. (2007). FEBS Letters, 581(21), 3915-3920.
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
T-cell dependent antigen specific in situ staining (Pe)
). Double staining (TUNEL): 19- warm the section in TBS 20- prepare a warm incubation box 21- set up the TUNEL mixture (see data sheet) 22- incubate 30 min at 37°C. 23- wash thrice in TBS-T 24- apply goat anti-FITC-HRP 1:200 25- wash twice
分析。 阈值：是循环开始3～15个循环的荧光信号的标准偏差的10倍，设定在扩增曲线指数增长期。 C(t)值：荧光信号（扩增产物）到达阈值时所经过的扩增循环次数。十、大肠杆菌感受态细胞（E.coli DH5α）制备 1. 前夜接种受体菌（DH5或DH10B），挑取单菌落于LB培养基中37 ℃摇床培养过夜（约16小时）； 2. 取1 ml过夜培养物转接于100 ml LB培养基中，在37 ℃摇床上剧烈振荡培养约
