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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
见标签
- 保质期：
2年
- 英文名：
Rapid Integrated Total Dietary Fiber
- 库存：
1
- 供应商：
上海经科化学科技有限公司
- 规格：
100 assays
Rapid Integrated Total Dietary Fiber Assay Kit介绍：
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Dietary Fiber |
| Assay Format: | Enzymatic |
| Detection Method: | Gravimetric/HPLC |
| Signal Response: | Increase |
| Limit of Detection: | 0.5 g/100 g |
| Total Assay Time: | ~ 3 h work (over 1-2 days) |
| Application examples: | Food ingredients, food products and other materials. |
| Method recognition: | AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard Method No. 185 and CODEX Method Type I |
- More rapid measurement - incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed)
- DF values for most samples are very similar to those obtained with AOAC Method 2009.01
- Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01*
- All reagents stable for > 2 years after preparation
- The method is consistent with the CODEX Alimentarius definition of dietary fiber
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Very competitive price (cost per test)
Rapid Integrated Total Dietary Fiber Assay Kit相关产品：
Rapid Integrated Total Dietary Fiber Assay Kit简介：
This test kit is suitable for the measurement and analysis of Total Dietary Fiber as per Codex Alimentarius definition,
updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time.
Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS4).
Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the
incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25 are no longer produced.
In this rapid, improved method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and
AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with
ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those
obtained with AOAC Methods 2009.01 and 2011.25.
The dietary fiber fractions that are measured with this method are:
1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and Low Molecular
Weight Soluble Dietary Fiber (SDFS; soluble dietary fiber which is soluble in the presence of 78% aqueous ethanol), or
2. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP; water soluble dietary fiber
that precipitates in the presence of 78% aqueous ethanol) and SDFS.
The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active
on other dietary fiber components such as
β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the
use of glycerol as an internal standard.
Rapid Integrated Total Dietary Fiber Assay Kit说明书(PDF)请向客服索取！
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- 作者
- 内容
- 询问日期
文献和实验1.Measurement of available carbohydrates, digestible, and resistant starch in food ingredients and products.
McCleary, B. V., McLoughlin, C., Charmier, L. M. J. & McGeough, P. (2019). Cereal Chemistry, 97(1), 114-137.
2.Definition and Analysis of Dietary Fiber in Grain Products.
McCleary, B. V., Cox, J, Ivory, R. & Delaney, E. (2019). “Cereal Grain-based Functional Foods", (Trust Beta and Mary Ellen Camire), CPI Group (UK) Ltd, pp. 103-126.
