http://www.alkami.com/ This 158 page PCR manual covers the following topics PCR Primer design PCR Methods PCR Polymerases PCR Variables PCR Troubleshooting Special PCR Topics Appendixes and a comprehensive Index
BioGuide - PCR http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html 魏茨曼基因组和生物信息学研究所的一个网页,介绍PCR的初步知识。 包括: What is PCR? What are some good reference books for PCR? How should I select a set of primers to use for PCR? Programs for designing PCR primers What is "Hot-start" PCR? What is AP-PCR or RAPD PCR? What is "Touchdown" PCR? Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?