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Important : Extract the DNA within one week of receiving samples.Samples that have been processed should be frozen to prevent degradation.Freeze samples between use each day. 1.Add 600 ml of 50 mM NaO ...

John Mundy Institute of Molecular BiologyCopenhagenDenmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#Footprint 1)probe: same as used for gelshift)isolated by isotacelectrophoresis w/out E ...

1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube.Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA.Mix immediatel ...

This is a brief overview of how a Southern blot (more formally called an DNA blot)is performed and what type of data you can obtain form one. Figure 1.Southern blots allow investigators to determine ...

1.Prepare or obtain Buffered phenolpH 8.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored. 2.Combine DNA sample with an equal volume of ...

1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Locate bands with a hand-held long-wave UV lamp. 2.Slice the gel with a razor blade above and below the bands of interes ...

i want to know how can i extract DNA from water microbe? -tanklm- -------------------------------------------------------------------------------- Standard DNA extraction proceedures should work fine ...

实验步骤： 1、Inoculate from an overnight grown in LB.从培养过夜的LB平板上挑取单菌落 。 2、Grow in 250 ml "SOB" at 18℃ until OD600 = 0.6.(0.3)接种于250ml SOB，18度培养至OD＝0.6。 3、On ice for 10 minutes.菌液置冰上10分钟。 4、Spin ...

1. Pick pl ...

Hi I looked for CpG islands within the promoter of my gene.MethPrimer and CpG plot analysis revealed that in this genes promoter region there were two big CpG islands at locations 280-603 and 693-859. ...

This is a modification of the procedure in Short Protocols (1-411-45) 1.Prepare a 50 ml liquid lysate: A.mix 2xlO8 E.coli cells with 100μl phage (from one picked plaque in 500μl SM)and 100μl ...

(adapted from Bruce A.RoeDepartment of Chemistry and BiochemistryThe University of OklahomaNormanOklahoma 73019 broe@ou.edu) Phenol extraction is a common technique used to purify a DNA sample (1).Typ ...

Materials: 1.TE solution o10 mM Tris (pH to 7.5) o1 mM EDTA (pH to 8.0 to dissolve) 2.Frozen agarose gel piece containing the desired DNA fragment Supplies: 1.Micropipetter and tips 2.Microcentrifuge ...

酶 最适温度 37℃%活性 Apa I 25℃ 10undefined ApeK I 75℃ n ...

1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10'5K4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10'5K4℃ 5.Wash 1X 40 ml 10% ...

I am trying to digest mouse genomic DNA for a southern blot. When digested with either HIndIII or NdeI I get a nice smear as expected. But when I digest with either speI or BglII (neither one of which ...

Isolation of DNA from Agarose Gels (Paper Slurry Method) This procedure isolates DNA from agarose gels by filtration through a filter-paper column.The column is made in a 500 µL tube from a slur ...

This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria we've tried. 1.Pellet cells from 10 ml overnight cultures in BHI or LB and wash in 5 ml ...

1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is correct for most applications. 2.Run out DNA fragments.For fragments greater than 500 bprun the xylene cyanol dye to the b ...

I have read some books but cannot find the answer.I think NaAc may increase the ionic strength to stabilize DNA duplexes.Is it right? Thanks. -freshman- ----------------------------------------------- ...