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Pre-sterilize two pairs of fine forceps one pair iris scissors and one blunt-ended 23 gauge needle. Take 3.5 day pregnant mouse kill and dissect out entire uterus using sterile technique. Place uteru ...

Before you start have ready the following: - Sterilize 2 pairs of curved forceps 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES with an 18 ...

Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra ...

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This method works well for 17.5 day mouse embryos. All stains should be made up fresh. Fix embryos in 90% ethanol for at least 1 week longer if possible. Prior to staining remove skin and viscera pa ...

本文介绍了均相荧光免疫测定的原理及方法。

The aggregation method for generating chimaeras as opposed to the microinjection technique is useful as it does not require expensive microinjection apparatus or sophisticated manipulative skills and ...

Blastocyst transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - the embryos should recavitate if they ha ...

本文系统介绍了 时间分辨荧光分析法（TRFIA）的优点、标记物、检测原理及参数。

本文对近年来发展起来的时间分辨免疫荧光技术作了一个扼要的介绍。内容包括该技术的原理、测定方法以及应用情况，并对该技术今后的发展作了展望。

一、载气钢瓶的使用规程 1 钢瓶必须分类保管，直立因定，远离热源，避免暴晒及强烈震动，氢气室内存放量不得超过二瓶。 2 氧气瓶及专用工具严禁与油类接触。 3 钢瓶上的氧气表要专用，安装时螺扣要上紧。 4 操作时严禁敲打，发现漏气须立即修好。 5 用后气瓶的剩余残压不应少于980 kPa。 6 氢气压力表系反螺纹，安装拆卸时应注意防止损坏螺纹。 二、减压阀的使用及注意事项（仪表同） 1在气相色谱分析 ...

GC9160系列化多功能气相色谱仪是目前国内最新型的气相色谱仪，它采用了最新的信息技术，在温度控制、电子电路通讯、仪器故障自诊、数据断电保护等方面均有独到之处。该款仪器针对企业、高校、厂矿等仪器不间断使用的特点，提出了“稳定高于一切”的设计思路，仪器在电路及气路方面严格采用国外著名气相色谱仪生产厂商的生产过程控制，使其无论在稳定性还是灵敏度指标都胜人一筹，尤其在气相色谱仪程 ...

1．开机前的准备：打开氮气、氧气瓶，并调分压表压力为0.6MPa。接通总电源。 2．打开氢气发生气电源开关。 3．检查各气路是否漏气。 4．开启主机与工作站，并使两者通迅。 4.1 确定各种抽需气体（N2、H2、Air）打开HP6890开关后，打开PC机并进入“Windows”，在“HP ChemStations”里选择“HP Confi ...

完整的实验方法请点击下面的链接下载： ...

DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载： ...

Mouse embryos staining including: in vitro transcription of riboprobe embryo fixation and permeabilization hybridization and post-hybridization washes immunodetection and development of signal. 完整的实验 ...

FGFR-AP-FGF Binding Assay PCR Assays for Knockout Mice RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of prote ...

The purity of the transgene DNA used for microinjection is critical for the successful production of transgenic founder mice. DNA impurities in the form of bacterial endotoxins or organic contaminants ...

1.Restrict DNA and gel purify Cut 100ug DNA removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of ...

一、摘要 目的：建立兔膀胱平滑肌细胞的分离、培养和鉴定的方法。方法：成年新西兰白兔两只（正常及梗阻各一只），采用酶法分离技术获得膀胱平滑肌细胞后于10%小牛血清的DMEM中培养，观察细胞形态和扩增情况，用爬片染色、电镜、蛋白质α-肌动蛋白（α-actin）鉴定细胞类型。结果：倒置显微镜下观察均呈“谷和峰”样结构、细胞爬片HE染色及电镜检查均证实为平 ...