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Human Genetics: Concepts and Applications(5th edition)Amazon网站介绍： http://www.amazon.com/Human-Genetics-Applications-Ricki-Lewis/dp/007246268XAuthor: Ricki LewisISBN: 007246268XPublisher: McGraw-Hill, 2002Format: PDF - 440 pagesSize: 79.5 MBook Desc ...

我在ExonPrimer（http://ihg.gsf.de/ihg/ExonPrimer.html）网页上设计引物，结果如下，不知道行不行？我是新手，我不明白这些参数，也看不懂，请各位多多指教。我要做的外显子是666bp的。Exon1: 666 bpOLIGO start len tm gc% any 3' rep seqLEFT PRIMER 225 20 58.19 50.00 2.00 2.00 12.00 GGTCTGTGTTTTGTTCCTGCRIGHT PRIMER 950 27 55.92 37.04 4. ...

http://www.ambion.com/techlib/tb/tb_502.htmlPublished siRNA Target SequencesApril 2003 - The significant increase in the rate of publication of siRNA target sequences has made it difficult to maintain a comprehensive list of these sequences. Therefore, we will no longer be upda ...

Dear ×××（某外国机构名称）,Thanks you for your kind assistance to our research project.I am an associate professor in ×××××××××（国内研究所或大学机构名称，最好不要用公司，以免被怀疑商业目的） and also member of the State Key Laboratory. And one of my projects focuses on ××××××（简单一句介绍实验目的）. Isolating cells seems to be a problem hard to g ...

文献的作者：Suzuki J, Fukuda M, Kawata S, Maruoka M, Kubo Y, Takeya T, Shishido T.题目：A rapid protein expression and purification system using Chinese hamster ovary cells expressing retrovirus receptor.期刊：J Biotechnol. 2006 Dec 1;126(4):463-74.PMID: 167660742.1. Cloning of EcoRAn ecotropic ...

1000 Genomes Project: ready to sequence07/05/2010Erin PodolakData from the three pilot projects recently conducted to determine how to sequence human genomes for the 1000 Genomes Project is now available, moving the project into its next phaseThe 1000 Genomes Project, an internatio ...

最新基因组学术语表New Genome GlossaryGenome GlossaryAAdenine (A): A nitrogenous base, one member of the base pair AT(adeninethymine).Allele: Alternative form of a genetic locus; a single allele for each locus isinherited separately from each parent (e.g., at a locus for eye color the allelemig ...

Preparation of BAC (Bacterial Artificial Chromosome) DNA with by Alkaline Prep Method1. Grow 250 ml of LB culture at 37 °C for 16 hours ( 250 rpm) with the appropriate antibiotic. Cultures can be started by 1) picking a single colony or 2) by splitting a subculture into 250 ml of culture (1 L Flasks). Colonies shou ...

可以肯定很多战友知道麻省理工学院的课件已经在网上全部公布，但有些战友可能不知道在哪里可以看到他们。下面提供支持在线浏览的生物学课件的网址给大家（并且这些教程是可以下载的）http://www.core.org.cn/OCW_CN/Biology/index.htm我想这对很多战友来说是不可多得的好教材。坦白来说，我把它当成一种财富，我近期已经阅读了他的遗传学教程，发现真的讲得很好，深入浅出的为我们讲述了遗传学的方方面 ...

我从网上看到的，与大家分享。点此下载Highly Efficient Ligation for Large InsertsOne of the major problems with the success of ligation reactions, and thus the cloning experiment as a whole, is ensuring the restriction digests are complete and the dephosphorylation of the vector termini was successf ...

Star ActivityIt has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed “star” ...

The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic formatis similar to other Qiagen products designed to purify nucleic acids: ion exchange columnsspecifically bind RNA which is purified by wash steps and subsequently eluted in aqueoussolution. The RNea ...

核酸相关技术动画集锦RT-PCR cartoon原文链接：http://www.kexue.com.cn/blog/user_content.aspx?id=13509Southern blot原文链接：http://www.kexue.com.cn/blog/user_content.aspx?id=13468DNA Fingerprinting原文链接：http://www.kexue.com.cn/blog/user_content.asp ...

基因打靶资料http://www.dxy.cn/bbs/post/view?bid=41&id=700550&sty=3&keywords=%BB%F9%D2%F2+%B4%F2%B0%D0http://www.dxy.cn/bbs/thread/1274625http://www.dxy.cn/bbs/post/view?bid=64&id=544760&sty=3&keywords=%BB%F9%D2%F2+%B4%F2%B0%D0http://www. ...

2008年初,由上海交通大学第一人民医院黄倩教授和美国科罗拉多大学李川源教授合作,携手北京病毒基因工程重点实验室吴小兵博士研究小组共同完成了目前国际上常用的病毒载体,象腺病毒\腺相关病毒\慢病毒(rAAV2/1, rAAV2, Ad5, Ad5/F35, Lentivirus)等病毒载体在视网膜上的特异性基因转导效率的研究,从而为特殊的视网膜疾病选用特殊的病毒载体进行基因治疗,提供了重要的见地认识!她们的结果发表在Current E ...

刚刚去BD网站，发现一个优惠活动，送BD绿色荧光蛋白表达载体，原价是6000千元的，赶快贴在这里，大家赶快去要呀，是个好东东！！Free AcGFP green monomer vector while supplies last!Commemorative Promotion: Ten Years in Fluorescent ProteinsDear Researchers,Over the past ten years, your feedback has helped Clontech beco ...

Gene (Ref)Accession #Position from ATGGene Target(Comments)Cell Line Testedß-actin (1) NM_001101972-994aatgaagatcaagatcattgcHuman HeLagamma-actin (1) NM_0016148-30aagagatcgccgcgctggtcaHuman HeLaAIB1 (estrogen receptor coactivator) (22) AAAGAC ...

【共享】英文）RNAi实验中双链短RNA（dsRNA）制备过程，本实验方法来自于加州大学Jim教授实验的.====请给出来源或连接.Procedure for the Generation of dsRNA for use in RNAi1. Design polymerase chain reaction (PCR) primers that will amplify 600-800 bp of sequence corresponding to the message of interest. Both primers shou ...

1）.Reference Manager 9.0实验准备阶段一般要查一些与实验相关的文献以便对自己所要做的课题的最新的进展有一个基本的了解，从而确定自己的实验策略。Manager 可以在线通过查找关键词搜索宋体; "Times New Roman"数据库中的专业资料,同时保存查找的资料为本地文件。资料内容和记录分上下两个屏幕，如有全文或想连接网络时敲键盘就可以到相应的全文文章和摘要。可以直接在WORD中查找资料，并插入引用。在文章中 ...

分子生物学实验人员软件解决方案分子生物学实验人员软件解决方案在互联网上，有很多软件可以解决对单个基因进行研究的分子生物学实验人员从立项到最后写论文的实际问题。本文提供了对相同作用的很多软件进行比较后推荐给一线实验人员使用的Windows应用软件。一、资料收集整理阶段。本阶段需要查找某个项目专题的文章,并写出对该专题的综述,以便对自己所要做的课题的现状有一个基本的了解。推荐软件：Reference Manager 9 ...