Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies
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Seed dormancy and germination are regulated by several plant hormones, such as abscisic acid, gibberellin, auxin (indole-3-acetic acid), ethylene, and brassinosteroid. Endogenous concentrations of a hormone are determined by the balance between biosynthesis and deactivation, and contribute to the regulation of physiological responses. Therefore, profiling of all hormones and their metabolites (hormonome) is a powerful approach to elucidate the regulatory networks of hormone metabolism. The methods involved in the use of liquid chromatography–electrospray ionization–tandem mass spectrometry to develop a high-sensitive and high-throughput hormonome platform are described in this chapter.
![///蛋白Recombinant Carcinus maenas Crustacean hyperglycemic hormones重组蛋白Crustacean hyperglycemic hormones [Cleaved into: CHH precursor-related peptide; CPRP); Crustacean hyperglycemic hormone; CHH)]蛋白](https://img1.dxycdn.com/p/s14/2024/0914/559/6161996242349384381.jpg!wh200)
![///蛋白Recombinant Glycine max 2S seed storage albumin protein重组蛋白2S albumin; GM2S-1; Napin-type 2S albumin 3) [Cleaved into: 2S albumin small chain; Aspartic acid-rich peptide; Lunasin); 2S albumin large chain; 8 kDa methionine-rich protein; 8 kDa MRP)]蛋白](https://img1.dxycdn.com/p/s14/2024/0914/429/2534610676379384381.jpg!wh200)
