Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves

The endogenous concentration of N -jasmonoyl-l -isoleucine (JA-Ile) is regulated by the balance between biosynthesis and deactivation and controls plant developmental processes and stress responses. Therefore, profiling of its precursors and metabolites is required to understand the mechanism by which the JA-Ile concentration is regulated. Also, other hormones, such as indole-3-acetic acid, abscisic acid, salicylic acid, and ethylene, have been suggested to interact with JA-Ile signaling. Profiling of these hormones and their metabolites should give us insights into their interaction mode. Liquid chromatography-electrospray ionization-tandem mass spectrometry has enabled us to develop a highly sensitive and high-throughput comprehensive quantification analysis of phytohormones.