【求助】最普通的HEK293细胞能包装慢病毒吗？
丁香园论坛
7010
请问大家，用最普通的HEK293细胞能包装慢病毒码？谢谢
从文献上看，已经有多篇文献报道了用HEK293来包装细胞。但是包装的效果可能没有其它293衍生细胞好。例如293FT来源于293细胞，293FT和293T细胞一样，都是转化了SV40大T抗原的293细胞的一个衍生物。这些细胞系具适宜的包膜和高表达反转录酶，能提高逆转录病毒的滴度。与293T细胞相比，293FT生长的更快，转染效率更高，因此其具有产毒滴度高的优点。
1 The 293 Cell Line is a permanent line established from primary embryonal human
kidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,
1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells
and participates in transactivation of some viral promoters, allowing these cells to
produce very high levels of protein.
2 The 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing
variant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia
3 The 293FT Cell Line is derived from the 293F Cell Line and stably
expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.
Expression of the SV40 large T antigen is controlled by the human cytomegalovirus
(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.
4 293T细胞表达E1A蛋白，SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。
kidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,
1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells
and participates in transactivation of some viral promoters, allowing these cells to
produce very high levels of protein.
2 The 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing
variant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia
3 The 293FT Cell Line is derived from the 293F Cell Line and stably
expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.
Expression of the SV40 large T antigen is controlled by the human cytomegalovirus
(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.
4 293T细胞表达E1A蛋白，SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。
能 我们实验室有人就这么做得
本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好伙伴
师兄微信号：shixiongcoming
