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        help:请各位高手帮忙看看我设计的SD大鼠caspase-3的引物

        丁香园论坛

        2698
        我最近用一在线的引物设计软件设计了一对SD大鼠caspase-3 的引物,不知行不,请高手多多帮忙.
        结果如下:
        Primer3 Output

        --------------------------------------------------------------------------------

        WARNING: Numbers in input sequence were deleted.

        No mispriming library specified
        Using 1-based sequence positions
        OLIGO start len tm gc% any 3' seq
        LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
        RIGHT PRIMER 1557 20 60.25 50.00 3.00 0.00 tagccttcaacaagccaacc
        SEQUENCE SIZE: 2484
        INCLUDED REGION SIZE: 2484

        PRODUCT SIZE: 216, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

        1 gggatcaaagcttagtgtcctgaggtgcggagcttggaacgcgaagaaaagtgaccatgg

        61 acaacaacgaaacctccgtggattcaaaatccattaataattttgaaacaaagactatcc

        121 atggaagcaagtcgatggactctggaatatatctggacagcagttacaaaatggattacc

        181 ctgaaatgggcttgtgtataataattaataataagaacttccataaaagcactggaatgt

        241 cagctcgcaatggtaccgatgtcgatgcagctaacctcagagagacattcatggccctga

        301 aatacgaagtcaggaataaaaatgaccttactcgtgaagaaattatggaattgatggata

        361 gtgtttctaaggaagatcacagcaaaaggagcagttttgtgtgtgtgattctaagtcatg

        421 gagatgaaggagtaatttttggaacgaacggacctgtggacctgaaaaaactaactagtt

        481 tcttcagaggcgactactgccggagtctgactggaaagccgaaactcttcatcattcagg

        541 cctgccgaggtacagagctggactgcggtattgagacagacagtggaactgacgatgata

        601 tggcatgccagaagataccagtggaggccgacttcctgtatgcttactctaccgcacccg

        661 gttactattcctggagaaattcaagggacgggtcatggttcatccagtcactttgcgcca

        721 tgctgaaactgtacgcgcacaagctggaattcatgcacatcctcactcgtgttaaccgga

        781 aggtggccacggaatttgagtccttctccctggacgccactttccatgcaaagaaacaga

        841 tcccgtgtattgtgtcaatgctcacaaaagaactgtacttttatcactaaaggaatgact

        901 gggagtggggtaggggcatgtttctgttttggtttttttttttggtttttgttttttgtt

        961 tttttttttttatttgaatgccaaatgagaaaactgtcagggagactttttttttcccct

        1021 ctcatttaaatcaaatccgatgttccaggtcgtcattgaacaataccactgcctgcaatg

        1081 cagccacaatacaatacctcagctttgatatcaggtagttgaaatgaaatttaattagga

        1141 acaaataaatgtcaataatggtacaaccatcatagaagaaattggaaagtcacactttgg

        1201 taattagagagatttggtgccactatgaatttgaaattacgaagcagtgatgatcttata

        1261 ccccctcctcacatctagaatgtcactttagttttcgttaagcataggaacgataatgtc

        1321 aagtggtatgtcttagaacttgaatccacgagcagagtcaaaggctggttttggaactga
        >>>>>>>>>>>>>>>>>>>>

        1381 tctgtggaaatgaagagttggagcactgtagcacacattatagctactggttttgtgaca

        1441 gttgaccaggtcacatatttattatttaagacaaaagtcgtgttttattattggaacaaa

        1501 aataactttatatgagaggaaaaccatgtgtgaacttggttggcttgttgaaggctacca
        <<<<<<<<<<<<<<<<<<<<

        1561 tccttcagtggtggacatgacgacagggtgctacgatccaccagcatttgtttcttactt

        1621 attcttatgcaaatcagcctaattttacagaccagcaaaatatctaaagagcgattatgc

        1681 agcagcctcaaattgttgactattagaatggaataaaggaattctacaggaacagaccat

        1741 aataccagctgtcagtcagagcgtaaggaaaggagaggtggcatctcctgtgattggaga

        1801 gaagatggtttgagccggagcagagccatgggcacgtcttcagaaaaggcctgctagttg

        1861 gacccaccttgtgagcactggtctgtgtggatgatgtccaagttaccacatgagactgaa

        1921 ccagatcagaagctcctgcaaaaaggctttcccagagtgctgagcatctcaagaaagttc

        1981 actggactccatgtacagagtctgtagcatgctgacttttctgttgaagtttacaatgaa

        2041 aggaaaatgatggtgatatttgtaccgtcactagaaatgaagggccctggcacacgggac

        2101 ttggaaagcatccagcaataggccctgcagcccatgtggaggagctgcagactggagtcc

        2161 acccctcctccccatcagggcgtctgtgctccaggcttccttaatcatggaggtgctgag

        2221 tcatcgctggctctccctgtgggtccagcatttcccataagcctccttattgcacaaaga

        2281 aaggaaggaactcacattataactgatagtaggttcttgtttagactggatttactgtta

        2341 actcttttctaagtaaatacagcatgtggtggtattttaaaatgtgttcccatgtgacaa

        2401 ttttataaaagtcagatatcttgcatttttatttcaaaacataaattcagatttaaaatt

        2461 aaaaaaaaaaaaaaaaaaaaaaaa

        KEYS (in order of precedence):
        >>>>>> left primer
        <<<<<< right primer

        ADDITIONAL OLIGOS
        start len tm gc% any 3' seq

        1 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
        RIGHT PRIMER 1550 20 59.73 45.00 3.00 1.00 caacaagccaaccaagttca
        PRODUCT SIZE: 209, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

        2 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
        RIGHT PRIMER 1554 20 60.66 50.00 3.00 0.00 ccttcaacaagccaaccaag
        PRODUCT SIZE: 213, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

        3 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
        RIGHT PRIMER 1546 20 59.19 45.00 3.00 0.00 aagccaaccaagttcacaca
        PRODUCT SIZE: 205, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

        4 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
        RIGHT PRIMER 1548 20 59.19 45.00 3.00 0.00 acaagccaaccaagttcaca
        PRODUCT SIZE: 207, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

        Statistics
        con too in in no tm tm high high high
        sid many tar excl bad GC too too any 3' poly end
        ered Ns get reg GC% clamp low high compl compl X stab ok
        Left 9195 0 0 0 698 0 2454 2424 693 0 1719 92 1115
        Right 9393 0 0 0 1124 0 2439 2270 32 0 1692 88 1748
        Pair Stats:
        considered 61917, unacceptable product size 61821, high any compl 88, ok 8
        primer3 release 1.0

        (primer3_www_results.cgi v 0.4)
        有同学说,有个引物评分,请问师兄我这对引物有多少分?谢谢!!!!!!
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