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        PCR from Tissue

        互联网

        1012

         

        1. collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH
        2. put in boiling H2 O for 30 sec (optimum may need to be determined for each type of tissue, for floral tissue 30 sec is fine)
        3. neutralize by adding 40 ml 0.25 N HCl and 20 ml 0.5 M Tris-HCl pH 8.0, 0.25% (v/v) Igepal CA-630
        4. boil for another 2 min
        5. use directly for PCR or store at 4°C for up to several weeks
        6. if stored at 4°C boil again for 2 min before the PCR

         

        Remarks:

        We don't have too much experience with this method. Mostly it seems to work though. However, I don't think it is safe enough for critical purposes (e.g., mapping).

        PCR方法相关产品:

        • 电泳设备
        • 紫外设备
        • 普通PCR仪
        • 定量PCR仪
        • PCR/RT-PCR/qPCR试剂
        • PCR引物
        • PCR试剂
        • PCR对照
        • 特异性PCR试剂盒
        • PCR克隆试剂盒
        • RNA
        • RNase检测/去除
        • RT-PCR试剂
        • RT-PCR标准品
        • 定量PCR试剂
        • 定量PCR标记
        • 总RNA分离纯化盒
        • PCR产物纯化
        • 核酸酶
        • 聚合酶
        • 反转录酶

         

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