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        DNA Sequencing by Hybridization with Arrays of Samples or Probes

        互联网

        373
        This chapter focuses on sequencing by hybridization (SBH), an advanced DNA sequencing technique first proposed in 1987 (1 ). SBH procedures determine DNA sequence information by screening DNA oligomers (typically 7-to 11-mers) for their ability to hybridize with target DNA. The set of overlapping oligomers that matches the target DNA is then used to assemble its sequence. The theory, practice, and history of SBH are reviewed in refs. 2 and 3 .
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