• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        PCR from Plant Tissue

        互联网

        1155

        1.protocol

        (1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH

        (2)put in boiling H2Ofor 30 sec (optimum may need to be determined for each type of tissue, for floral tissue 30 sec is fine)

        (3)neutralize by adding 40 ml 0.25 N HCl and 20 ml 0.5 M Tris-HCl pH 8.0, 0.25% (v/v) Igepal CA-630

        (4)boil for another 2 min

        (5)use directly for PCR or store at 4°C for up to several weeks

        (6)if stored at 4°C boil again for 2 min before the PCR

        2.Remarks

        We don't have too much experience with this method. Mostly it seems to work though.However, I don't think it is safe enough for critical purposes (e.g., mapping).

        3.Materials

        Reagent Supplier Cat.-#
        Igepal CA-630 Sigma I-3021

        4.Reference

        Klimyuk et.al., 1993, Plant J. 3:493-494

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序