Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on Chromosome 11p15.5

Lung cancer is one of the most common cancers afflicting the citizens of developed countries (1 ). While the lung is a complex tissue composed of over 40 different cell types, the most common lung cancers, large cell lung carcinoma, small cell lung carcinoma (SCLC), squamous cell carcinoma, and adenocarcinoma, are all thought to arise from bronchoepithelial cells (2 ). Normal human cells are not easily coerced into becoming cancerous as numerous mutations are necessary to subvert the cellular processes that ensure the fidelity of DNA replication and that limit cell growth and proliferation (3 ).