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PCR Procedure for the Isolation of Trinucleotide Repeats

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Microsatellites, also referred to as short tandem repeats (STR) or simple sequence repeats (SSR), are highly polymorphic and abundant sequences dispersed throughout most eukaryotic nuclear genomes (1 -3 ). In recent years, microsatellites have been used for linkage map construction, population genetics, molecular evolution studies, forensic sciences, and as parentage testing markers (4 ,5 ).
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