AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation
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If a sample has a comparatively even surface and is fixed on a sample stage, atomic force microscopy (AFM) will give a clear image of the surface structure at subnanometer level (1 ,2 ). Because a sperm head is flat and can be attached on the slide glass firmly after it is fixed, we consider that AFM is the competent tool for the study of the sperm surface structure.
