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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse R-Spondin 1 / RSPO1 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25880.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1580.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3980.0 |
蛋白名称:RSPO1蛋白, RSPO1 protein
蛋白构建:A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.
表达宿主:CHO Stable Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/ml in the presence of 5 ng/mL recombinant mouse Wnt3a.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 21
蛋白分子量:The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_619624.2
蛋白氨基酸序列:Met1-Gln265
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Cheng, CW; et al. Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet. Cell, PMID: 31442404
3, Gao, L; et al. Sensitive Quantitative In Vivo Assay for Evaluating the Effects of Biomolecules on Hair Growth and Coloring Using Direct Microinjections into Mouse Whisker Follicles. Biomolecules, PMID: 37509112
4, Xu, H; et al. ILC3s promote intestinal tuft cell hyperplasia and anthelmintic immunity through RANK signaling. Science immunology, PMID: 40378237
Identification of Peptide Features in Precursor Spectra Using Hardklör and Krönik
Du, P. and Angeletti, R.H. 2006. Automatic deconvolution of isotope‐resolved mass spectra using variable selection and quantized peptide mass distribution. Anal. Chem. 78:3385‐3392.
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye
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