Isolation of Plasmids from E. coli by Boiling Lysis
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The boiling lysis procedure (1 ) is quick to perform and, therefore, especially suitable for screening large numbers of small-volume Escherichia coli cultures. It is described with different adaptations in a variety of protocol books (2 ,3 ). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.
