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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Escherichia coli T4 DNA ligase protein
- 亚型:
Rabbit IgG
- 形态:
液体
- 保存条件:
-20℃ to -80℃
- 克隆性:
兔多抗
- 适应物种:
E. coli
- 保质期:
12个月
- 目录编号:
40066-RP01
- 级别:
免疫学试剂
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
ELISA: 1:1000-1:2000
- 靶点:
T4ligase
- 抗体英文名:
Escherichia coli T4 DNA ligase Antibody, Rabbit Pab
- 抗体名:
Escherichia coli T4 DNA ligase Antibody, Rabbit Pab
- 规格:
20 µL/100 µL
| 规格: | 20 µL | 产品价格: | ¥800 |
|---|---|---|---|
| 规格: | 100 µL | 产品价格: | ¥2299 |
抗体种属:E. coli
抗体靶点:T4ligase
抗体应用:ELISA
抗原货号:0
抗原描述:Recombinant Escherichia coli T4 DNA ligase protein
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体制备:Produced in rabbits immunized with purified, recombinant Escherichia coli T4 DNA ligase. Total IgG was purified by Protein A affinity chromatography.
抗体特异性:Escherichia coli T4 DNA ligase
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
DNA Ligation and Escherichia coli Transformation
The generation of recombinant clones usually involves joining together covalently (also called ligating) two or more restriction fragments. This can be done with the enzyme DNA ligase. There are two major sources of the enzyme: Escherichia, coli
【交流】关于NEB的T4 DNA连接酶和快速连接试剂盒的常见问题及解答
液: 1X T4 DNA Ligase Buffer: [50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, 25 µg/ml BSA, (pH 7.5 @ 25°C)]。推荐DNA浓度(0.1 - 1 µM 的5′末端)。 使用注意:ATP是反应必须的辅因子。这一点与要求NAD作辅因子的大肠杆菌DNA连接酶不同。 如在反应体系中补加1 mM的ATP,连接反应还可以在NEB提供的四种限制性内切酶缓冲液(NEBuffers)或在T4 DNA多聚
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