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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Escherichia coli T4 DNA ligase protein
- 亚型:
Rabbit IgG
- 形态:
液体
- 保存条件:
-20℃ to -80℃
- 克隆性:
兔多抗
- 适应物种:
E. coli
- 保质期:
12个月
- 目录编号:
40066-RP01
- 级别:
免疫学试剂
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 靶点:
T4ligase
- 抗体英文名:
Escherichia coli T4 DNA ligase Antibody, Rabbit Pab
- 抗体名:
大肠杆菌T4连接酶兔多抗
- 规格:
20.00 µL/100.00 µL/200.00 µL/400.00 µL
| 规格: | 20.00 µL | 产品价格: | ¥800.0 |
|---|---|---|---|
| 规格: | 100.00 µL | 产品价格: | ¥2299.0 |
| 规格: | 200.00 µL | 产品价格: | ¥1699.0 |
| 规格: | 400.00 µL | 产品价格: | ¥2399.0 |
抗体种属:E. coli
抗体靶点:T4ligase
抗体应用:ELISA
抗原描述:Recombinant Escherichia coli T4 DNA ligase protein
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体制备:Produced in rabbits immunized with purified, recombinant Escherichia coli T4 DNA ligase. Total IgG was purified by Protein A affinity chromatography.
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
DNA Ligation and Escherichia coli Transformation
The generation of recombinant clones usually involves joining together covalently (also called ligating) two or more restriction fragments. This can be done with the enzyme DNA ligase. There are two major sources of the enzyme: Escherichia, coli
Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
can be applied to a few common Escherichia coli hosts (e.g., BL21(DE3), DH5α, JM109, TOP10). The protocol includes three steps: (a) linear DNA fragments (i.e., the insert DNA and the vector backbone) with two overlap ends were generated by regular high-fidelity
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