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        培养细胞应该养成的一些好习惯

        互联网

        1976

        1. Wash hands before starting

        2. Keep work area free of clutter

        3. Correctly, legibly and informatively label all reagents including flasks and dishes with contents and date of preparation

        4. Sanitize material such as pipet cans with 70% ethanol before taking them inside the hood

        5. Put all material in hood before starting work (unless it clutters the work area)

        6. Direct coughing, sneezing and speaking away from the hood

        7. Do not put material on the edge of the hood over the vents

        8. Dispose sharps such as pipetman tips, glass pipettes, needles and blades in the sharps container

        9. Do not dispose non-biological material such as paper and liquids in the disposal bags

        10. At end of work, sanitize the aspirator tubing with 70% ethanol while the vacuum is on

        11. Examine cultures and media daily for gross microbial contamination

        12. Do not continously use antibiotics in culture medium

        13. Remember to turn off Pipet Aid

        14. Turn on UV light at end of work

        15. Rinse emptied glass bottles with tap water and leave some water inside

        16. Work without antibiotics if possible

        17. Get cells from reliable source

        18. Keep records

        19. Validate origin of cells

        20. Check for mycoplasma when importing cells

        21. When discarding liquid waste into beakers, place a sterile funnel over the beaker and discard into it.

        22. When fitting a pipette intoa pipetting device, hold the pipette near the top, with its tip pointing away from you, inside the hood and away from any surface.

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