发育生物学
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In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos -
Mini-Chamber for regulating gaseous environment during culture (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device -
Cell number counting in preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number -
TUNEL procedure for preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A protocol for detecting cell death using TUNEL. - Whole mount TUNEL analysis of Xenopus embryos (Peter Vize, Xenopus Molecular Marker Resource)
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Lineage Analysis Using Retrovirus Vectors (Cepko Lab, Department of Genetics, Harusr/locald Medical School and Howard Hughes Medical Institute,)
Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.
