Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA

The quantification of human immunoglobulin G (IgG) and recombinant derivatives is required for many applications in research and diagnostics. Particularly in research, robust and simple protocols are necessary to determine the concentration of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion proteins even in the presence of bovine serum proteins. The high sensitivity of the human IgG/Fc capture ELISA allows the detection of less than one nanogram of human IgG per millilitre.