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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IgG1-Fc Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg/200.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥7600.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥1660.0 |
| 规格: | 200.00 µg | 产品价格: | ¥2310.0 |
蛋白名称:Human IgG1-Fc Protein (103 Cys/Ser)
蛋白构建:A DNA sequence encoding the human IgG1 Fc region (P01857-1) (Glu99-Lys330) (one aa mutation, 103 Cys/Ser) was expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to bind human CD16a-His (Cat:10389-H08H1) in a functional ELISA. 2. Measured by its ability to bind human CD16a-AVI-His (Cat:10389-H27H) in a functional ELISA. 3.Measured by its ability to bind human CD16a-His (Cat:10389-H08H) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 99
蛋白分子量:The recombinant human IgG1 Fc consists of 232 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of rhFc is approximately 32 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P01857-1
蛋白氨基酸序列:Glu99-Lys330,C103S
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Gao, Q; et al. The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesis. Nature communications, PMID: 28589943
3, Chen, J; et al. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development. Proceedings of the National Academy of Sciences of the United States of America, PMID: 28827364
4, Li, J; et al. Tumor necrosis factor ligand-related molecule 1A maintains blood-retinal barrier via modulating SHP-1-Src-VE-cadherin signaling in diabetic retinopathy. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, PMID: 34679191
5, Marshall, MJE; et al. Development of C1q Affinity Chromatography for the Study of C1q-IgG Interactions. Journal of immunology (Baltimore, Md. : 1950), PMID: 37093649
6, Park, JS; et al. Production of recombinant human IgG1 Fc with beneficial N-glycosylation pattern for anti-inflammatory activity using genome-edited chickens. Communications biology, PMID: 37264071
人免疫球蛋白G Fc 片段(Fcγ)酶联免疫分析(ELISA)
人免疫球蛋白G Fc 片段(Fc γ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中免疫球蛋白G Fc 片段(Fc γ) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人免疫球蛋白 G Fc 片段 (Fc γ ) 水平。用纯化的 人免疫球蛋白 G Fc 片段 (Fc γ ) 抗体 包被微孔板,制成固相抗体,往包
层细胞高表达FcRn,可介导母体血中IgG进入胎儿血循环;通过肠上皮的FcRn,可将母体初乳中IgG转运到新生儿血循环。将人IgG通过肠道上皮细胞转运到肠腔结合相应的抗原,然后又将IgG/抗原复合物通过肠上皮细胞屏障又转运回黏膜固有层,被DC所加工,并提呈给局淋巴样组织中CD4+T细胞,表明IgG也是黏膜表面重要的免疫分子。 ②Fcα/μR,一种新的既可结合IgA又可结合IgM的Fc受体,人Fcα/μR基因与pIgR相邻。成熟Fcα/μR为单链跨膜蛋白,胞膜外区有3个结构
应答。 FcεRⅡ 即CD23,又称BLAST-2,Ⅱ型膜蛋白,胞膜外区C端含有1个C型凝集素样结构域,并形成三聚体。FceRⅡ(CD23)与FceR I无任何同源性。已发现有FctRⅡa和FcaRⅡb两种异型。FcsRⅡa异型仅表达于B细胞,而FctRⅡb异型可表达于其他多种细胞。 CD23分子通过蛋白酶水解可形成37 kDa可溶型CD23,并可进一步水解为33kDa、29kDa、25kDa和16kDa片段,所有这些片段均保留了C端的外源凝集素“头”样结构域,并具有与IgE结合的能力
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