Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression

RNAi can be an effective means to regulate endogenous gene expression in maize and as such represents an important reverse genetics tool. This approach involves designing a transgenic construct that creates a double stranded RNA (dsRNA) upon transcription, and introducing the transgene into maize plants. Transgenic lines bearing such a construct can be generated and characterized that are deficient in the gene of interest. Some variability has been observed in the efficiency of this technique, and there are several important aspects to consider. Herein, a basic protocol for using transgene-induced RNAi in maize is described, and some important considerations that can influence the success of this approach are discussed.