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        Construction and Use of a Bottom-Up HAC Vector for Transgene Expression

        互联网

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        Recent technological advances have enabled visualization of the organization and dynamics of local �chromatin structures; however, the global mechanisms by which chromatin organization modulates gene regulation are poorly understood. We designed and constructed a human artificial chromosome (HAC) vector that allows regulation of transgene expression and delivery of a gene expression platform into many vertebrate cell lines. This technology for manipulating a transgene using a HAC vector could be used in applied biology.
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