Single Worm PCR

Lysis Buffer:

50 mM KCl

10 mM Tris pH 8.3

2.5 mM MgCl2

0.45% NP-40 (IGEPAL)

0.45% Tween-20

0.01% Gelatin

Proteinase K:

20 mg/ml

10X PCR Buffer:

100 mM Tris, 500 mM KCl, 15 mM MgCl2 pH 8.3

dNTP mix:

25 mM/each

primers:

5-10 uM

Taq Polymerase:

approx 5U/ul