Single Worm PCR
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<center> <h1> Single Worm PCR</h1> </center>
Reagents:
| Lysis Buffer: | 50 mM KCl 10 mM Tris pH 8.3 2.5 mM MgCl2 0.45% NP-40 (IGEPAL) 0.45% Tween-20 0.01% Gelatin | 
| Proteinase K: | 20 mg/ml | 
| 10X PCR Buffer: | 100 mM Tris, 500 mM KCl, 15 mM MgCl2 pH 8.3 | 
| dNTP mix: | 25 mM/each | 
| primers: | 5-10 uM | 
| Taq Polymerase: | approx 5U/ul | 












