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        DNA标记

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        4856

         

        DNA 标记(主要内容如下)

         

        •   DNA Labeling by Nick Translation
        •   Random Primed Labeling
        •   End-Labeling
        •   Purification of Labeled DNA
        •   Non-isotopic Labeling
        •   Others

        DNA Labeling by Nick Translation

        §         DNA Labeling by Nick Translation (Ambros Lab)

        §         Nick Translation (Worthington)

        §         Nick Translation

        §         Nick Translation (Promega)

        §         Nick Translation (Life Technologies)

        Random Primed Labeling

                 Random Primer Labeling (Karl Clark)
        Prepare radioactively labeled DNA strands for the detection of target DNA or RNA sequences in usr/localious applications including Southern and northern blot hybridization. Detailed protocol for labeling, purification and determination of incorporation

                 Random Primed Labelling with P32 (Molecular Genetics Network Lab)

                 Random Primer Labeling (Worthington)

                 Random Primer Labeling (Crawford Lab)

                 Random primer labeling (Amersham Pharmacia)

                 Random Primer  DNA Labeling (Life Technologies)

                 Random Primer DNA labeling (Fermentas)
        Labeling of DNA by random oligonucleotide-primed synthesis

                 Random Hexamer Labeling of cDNA Probes (PMCI Research)

        End-Labeling

                 5'-End Oligo Fluorescence Labeling (Amersham Pharmacia)
        Labeling of  oligonucleotides with a single fluorescein residue at the 5' end

                 5' end labeling of oligonucleotides with gamma-32 P-ATP (Petra Klaff)
        Labeling and purification method in great detail

                 3'-end Labeling (Promega)

                 DNA Labeling using Klenow Fragment I (Lazo Lab)

                 End-labeling of DNA fragments
        Single end or double ends, can also be used for RNA

                 Labeling of Oligonucleotides (PMCI Research)
        Label oligo with polynucleotide kinase. 

                 Alkaline phosphatase labeling of oligos (Promega)
        Provides useful guide for preparing phosphatase conjugated oligos

                 Kinase End-Labeling of DNA (Roe, OU)

                 Endlabeling of DNA fragments with Klenow polymerase (Molecular Genetics Network Lab)

                 5'-end Labeling (Amersham Pharmacia)

                 Fill in Labeling of DNA (Mike A. Dyer)

                 5'-End Labeling (Fermentas)
        T4 polynucleotide kinase  labeling (ds and ss DNA or RNA; protruding, recessed and blunt 5'-ends) for (1)end labeling of DNA or RNA probes in DNA hybridization and DNA footprinting experiments, etc; (2)obtaining labeled molecular weight markers;(3)obtaining labeled molecular weight markers; (4)labelling of primers for sequencing; (5)addition of 5'-phosphates to oligonucleotides to allow subsequent ligation.

                 Oligonucleotide labeling with 32 P (Hahn Lab)
        T4 polynucleotide kinase labeling 

        Purification of Labeled DNA

        §         5' end labeling of oligonucleotides and purification (Petra Klaff)
        Labeling and purification method (ethanol and CTAB in great detail

        §         Oligo Purification on Acrylamide Gels (Botwell Lab)
        Purification of labeled oligos

                 Sephadex G-50 Spin Column (gopher://iubio.bio.indiana.edu/ )
        For purifying labeled probe

                 Spin Column chromatography (Schimenti Lab)
        Used to removed unincorporated nucleotides from labeling reactions.

                 Oligonucleotide Purification by PAGE and DEAE (Hahn Lab)

                 PAGE Purification of Oligonucleotides (Life Technologies)
        Protocol for PAGE Purification of Oligonucleotides Protocol for PAGE Purification of Oligonucleotides Oligonucleotides contaminated by significant amounts of aborted synthesis products (e.g., n-1,...

        Non-isotopic Labeling

                 PCR DNA Biotinylation (KPL)
        A rapid method for biotinylating DNA probes through incorporation of biotin-N4 -dCTP via a thermostable DNA polymerase in the polymerase chain reaction

                 Determination of efficiency of digoxigenin labelling (Molecular Genetics Network Lab)

                 Non-isotopic Labeling (Gottschling Lab)
        Protocols for preparing DNA, RNA probe and molecular marker

        Others

                 PCR Products Labeling (Amersham)
        Guidelines for preparation of radioactively-labeled PCR products for use as probes

         

         

         

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