Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons
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Dopaminergic neuronal cell degeneration is the principal characteristic feature of the neuropathology of Parkinson’s disease. Cultures of mesencephalic neurons are widely used as a source of dopaminergic neurons for the study of mechanisms implicated in dopaminergic cell death and for the evaluation of potential dopaminergic neuroprotective agents, including neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat mesencephalic cell cultures and their application to evaluating the neuroprotective action of brain-derived neurotrophic factor.
