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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB)品名简称:
Recombinant Mycobacterium tuberculosis esxB protein货号:
CSB-EP363658MVZ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
esxBUniprot No.:
P9WNK4别名:
10KDA culture filtrate antigen CFP-10 Secreted antigenic protein MTSA-10种属:
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.7 kDa表达区域:
2-100aa氨基酸序列:
AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M.J. Bacteriol. 184:5479-5490(2002)研究领域:
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文献和实验Antigen Design Sera Purification Tech Sheet
of the predictive methods may result in a success rate as high as 86 in predicting antigenic determinants9,10. Once the protein region of interest has been identified, the length of the peptide must be selected. There are two differing thoughts on the topic
Antigen Design Sera Purification Tech Sheet
. To be sucessful, none of the algorithms should be used alone. Combined use of the predictive methods may result in a success rate as high as 86% in predicting antigenic determinants9,10 . Once the protein region of interest has been identified
Transient transfection into 293T cells
, dust-like precipitate visible. After incubation, rinse once and change to medium without chloroquine, again 10ml/dish. 5. Harvest cells, or culture supernatant, 48-72 h after transfection. For secreted protein, you can change media once (at day
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