石蜡加填充剂 Paraffin Embedding

互联网2013-12-26

1932

Paraffin Embedding 石蜡加填充剂

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.

1. Materials

Sakura FineTek V.I.P. tissue processor (Sakura FineTek Inc.)
70%, 80%, 95%, 100% ethanol
Paraffin wax (Paraplast; melting point 58°C)
Xylenes, Reagent Grade (Sigma)
Embedding molds

2. Method

Fix tissue overnight in 70% EtOH at 4°C.
Place the tissue in the tissue processor and process with the times and temperatures described in Table 1 below.
Embed the specimen in paraffin and block.
Cut sections at 5 to 8 µm thickness.
Both paraffin blocks and sections may be stored at or below room temperature.
TIP: Tissue processors in standard histopathology laboratories generally include formalin-fixation as the first two steps in the paraffin infiltration procedure. Make certain these steps are avoided when processing tissue intended for molecular profiling.

<center> <blockquote> </blockquote> Table 1 <table> <caption> </caption> <tbody> <tr> <th> Station </th> <th> Solution </th> <th> Concentration </th> <th> Time(min) </th> <th> Temp(°C) </th> </tr> <tr> <td> 1 </td> <td> Ethanol </td> <td> 70% </td> <td> 30 </td> <td> 40 </td> </tr> <tr> <td> 2 </td> <td> Ethanol </td> <td> 80% </td> <td> 30 </td> <td> 40 </td> </tr> <tr> <td> 3 </td> <td> Ethanol </td> <td> 95% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 4 </td> <td> Ethanol </td> <td> 95% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 5 </td> <td> Ethanol </td> <td> 100% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 6 </td> <td> Ethanol </td> <td> 100% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 7 </td> <td> Xylenes </td> <td> 100% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 8 </td> <td> Xylenes </td> <td> 100% </td> <td> 45 </td> <td> 40 </td> </tr> <tr> <td> 9 </td> <td> Paraffin </td> <td> </td> <td> 30 </td> <td> 58 </td> </tr> <tr> <td> 10 </td> <td> Paraffin </td> <td> </td> <td> 30 </td> <td> 58 </td> </tr> <tr> <td> 11 </td> <td> Paraffin </td> <td> </td> <td> 30 </td> <td> 58 </td> </tr> <tr> <td> 12 </td> <td> Paraffin </td> <td> </td> <td> 30 </td> <td> 58 </td> </tr> </tbody> </table> </center> <center> </center>
上一篇：Microdissection Overview 显微解剖下一篇：Low-melt Polyester Embedding

关于丁香通

公司信息

个人用户

企业机构

无忧采购轻松科研

提问

扫一扫

实验小助手

扫码领资料

反馈

TOP

打开小程序