Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining

Adenovirus (Ad)-infected cells have been used extensively as a model system for studying the expression of eukaryotic genes and were instrumental in elucidating steps m the production of RNA. Much of this work involved genetic and molecular analyses of viral gene expression. Recent advances m methodologies for detecting nucleic acid and protein components of cells has triggered a renaissance in the study of cellular organization. Cell biologists interested in understanding functional organization of gene-expression activtties are again turning to Ad-infected cells as an important model system (reviewed in ref. 1 .