• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        DNA的诱变和甲基化

        互联网

        1494

         

                 In Vitro Mutagenesis Using Altered Sites (Bowtell Lab) 

        • In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab)

                 Site-directed Mutagenesis using PCR (Lazo Lab)
        PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.

                 Site-directed mutagenesis (Dr. Chastain)

                   Site Directed Mutagenesis Using the Ung-Dut System (Bowtell Lab)

                 Oligonucleotide-directed mutagenesis (Kunkel method) (Jeff NEwman)

                 Site-directed Mutagenesis (Promega)
        For generation and selection of oligonucleotide-directed mutants

                 Site Directed Mutagenesis Using the Ung-Dut System (PMCI Research)

                 Nested Deletions (Bowtell Lab)
        Nested Deletions using exonuclease-III and mung bean nuclease

                 Deletion Mutagenesis (Promega)
        For construction of plasmid or M13 subclones containing progressive unidirectional deletions of  inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation,  transformation...

                 ExoIII/S1 Deletion (Fermentas)
        For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.

                 Exo/S1 Deletion Series (Vesicle Trafficking)

         

        •  

        Four Primer Mutagenesis (PDF) (Templenton Lab)
        A PCR-based mutagenesis, based on Sarkar and Sommer, but with no ligation required. 

                 Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (PMCI Research)

                 In vitro Mutagenesis Using Altered Sites (PMCI Research)

                 Unidirectional deletions (erase-a-base) (Crawford Lab)

         

        •  

        Design of Mutagenic Oligonucleotides (Promega)

        Methylation analysis

                 Bisulfite Treatment of DNA (Issa Lab)

                 Methylation-Sensitive PCR (MSP) (Issa Lab)

                 Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab)

                 Southern Blot Analysis (Issa Lab)

                 Methylated CpG Island Amplification (MCA) (Issa Lab)

                 DNA-Methyltransferase Assay (Issa Lab)

                 Bisulfite Modification (TTO)
        Modify DNA with bisulfite for methylation analysis

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序