• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Analysis of Th1/Th2 T-Cell Subsets

        互联网

        683
        Specific immune responses are mediated by activated CD4+ T-helper (Th) cells. Two major subsets, denoted Th1 and Th2, have been identified that are characterized by their distinctive cytokine secretion pattern and associated effector functions. The signature cytokines of Th1 and Th2 cells are interferon-γ and interleukin-4, respectively. Because of the dominant role of Th cells in directing specific immunity, the analysis of Th subsets by means of determining their signature cytokines has contributed greatly to the progress that has been made in recent years in the understanding of protective as well as pathogenic immune responses. Several methods, such as reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, ELISpot, and intracellular flow cytometric analysis are used for the analysis of T-cell cytokines and, thus, of Th subsets. Here, we briefly discuss the advantages and disadvantages of these methods and describe in detail a standard protocol for the analysis of human Th subsets by means of detection of cytoplasmic cytokines by flow cytometry.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序