Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase

Oxidases remove hydrogen from substrates, transferring it to molecular oxygen to generate hydrogen peroxide or water. Since oxidases catalyze fundamental synthetic transformations, including alcohol-aldehyde and aldehyde-carboxylic acid conversions, they have attracted attention as targets of directed evolution for further improvement (1 ,2 ). The co-product hydrogen peroxide can be detected by various methods (3 ) and provides a basis for screening oxidases in a high throughput fashion.