Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay

The common cardiovascular, respiratory, gastrointestinal tract, renal, and immune diseases share common etiologies, because they are multifactorial in origin, with both genetic and environmental components contributing to their clinical phenotype. Recently, the necessity for the identification and analysis of the inheritance of single-nucleotide polymorphisms (SNPs) in these complex diseases has become apparent. First, to identify genes contributing to susceptibility to these diseases, and second, to determine whether some regulatory SNPs may influence the severity or course of a disease.