Ribonuclease A重组蛋白|Recombinant Human RNase A / Ribonuclease A / RNASE1 Protein (His Tag)
- ￥1790 - 29480
- Sino Biological
- 北京
- 13468-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human RNase A / Ribonuclease A / RNASE1 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/20.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥29480.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥1790.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
蛋白名称：Ribonuclease A蛋白, Ribonuclease A protein
蛋白构建：A DNA sequence encoding the human RNASE1 (P07998) (Met1-Thr156) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to hydrolyze yeast RNA(Sigma,Catalog # R9001). One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37°C and pH 7.0. The specific activity is >2.5×106 unit/mg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Lys 29
蛋白分子量：The recombinant human RNASE1 comprises 139 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 29 ,26 and 22 kDa in SDS-PAGE under reducing conditions
蛋白NP号：P07998
蛋白氨基酸序列：Met1-Thr156
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
Ribonuclease Protection Assaycontributed by James McCaughern-Carucci, Yale UniversityMost RNase Protection protocols require an overnight hyb with numerous subsequent clean-up steps. This method requires a maximum hyb of four hours, and the clean
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