Recombinant Human RNase A / Ribonuclease A / RNASE1 Protein (His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
100 µg
人 Ribonuclease A蛋白|Ribonuclease A protein|Ribonuclease A(Human, C-His)(产品说明)
蛋白名称:Ribonuclease A蛋白, Ribonuclease A protein
蛋白构建:A DNA sequence encoding the human RNASE1 (P07998) (Met1-Thr156) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to hydrolyze yeast RNA(Sigma,Catalog # R9001). One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37°C and pH 7.0. The specific activity is >2.5×106 unit/mg.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Lys 29
蛋白分子量:The recombinant human RNASE1 comprises 139 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 29 ,26 and 22 kDa in SDS-PAGE under reducing conditions
蛋白NP号:P07998
蛋白氨基酸序列:Met1-Thr156
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Lee, HH; et al. Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation. Nature communications, Pubmed: 33986289