Detection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney Proteins

Nitric oxide (NO) is a diatomic free-radical product of mammalian cells that has diverse and important physiological functions, including the regulation of renal, cardiovascular, pulmonary, gastrointestinal, neuronal, inflammatory, and immune systems. At the cellular level, NO is a pivotal modulator of proliferation, differentiation, migration, and programmed cell death. These actions of NO are engendered by covalent chemical addition of NO (or NO-derived species) to protein targets.