PCR clean up using 3M sodium acetate and chilled absolute ethanol

互联网2013-09-06

1180

For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.

2 ul of 3M sodium acetate (NaOAc) pH 4.5
40 ul chilled absolute ethanol.

Transfer the PCR product (20ul) into the tube containing the mixture.

Vortex the tube and then store it at -20 for 30-50 min.

Spin for 30 min at maximum speed 14.000 rpm.

Rremove the supernatant carefully and don''t disturb the pellet. ( Do not touch the bottom of the tube)

Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min.

Remove the supernatant and then dry the pellet using vacuum centrifuge. (do not apply heat)

Resuspend the pellet in 30 ul in sterile water.

相关产品推荐

Gel & PCR Clean Up Kit

￥598

Thermo Scientific ABsolute qPCR Plate Seals 粘附型荧光定量封板膜 AB1170

￥554

醋酸钠标准溶液，127-09-3，0.3M，阿拉丁

￥247.90

[(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-6-(2-pyridin-2-ylethylcarbamoyloxy)-5,6,6a,8,9,10-hexahydro-2H-benzo[f]chromen-5-yl] acetate，Moligand™，阿拉丁

￥3999.90

乙醇中吡虫啉溶液，138261-41-3，100μg/mL in Ethanol，不确定度3%，阿拉丁

￥82.90

关于丁香通

公司信息

个人用户

企业机构

无忧采购轻松科研

提问

扫一扫

实验小助手

扫码领资料

反馈

TOP

打开小程序