PCR clean up using 3M sodium acetate and chilled absolute ethanol

互联网2013-09-06

1210

For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.

Transfer the PCR product (20ul) into the tube containing the mixture.

Vortex the tube and then store it at -20 for 30-50 min.

Spin for 30 min at maximum speed 14.000 rpm.

Rremove the supernatant carefully and don''t disturb the pellet. ( Do not touch the bottom of the tube)

Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min.

Remove the supernatant and then dry the pellet using vacuum centrifuge. (do not apply heat)

Resuspend the pellet in 30 ul in sterile water.

相关产品推荐

Gel & PCR Clean Up Kit

￥598

Thermo Scientific ABsolute qPCR Plate Seals 粘附型荧光定量封板膜 AB1170

￥554

Ethyl 2-(2-Benzothiazolyl)acetate

￥60

EIF3M 多克隆抗体 11423-1-AP

￥1350

乙醇含量检测试剂盒，用于对样本中Ethanol含量检测，Ethanol Content Detection Kit

￥1026