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        Messenger RNA Fractionation on Neutral Sucrose Gradients

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        The separation of RNA on the basis of size by sucrose gradient fractionation is a technique frequently employed in a cloning strategy (1 3 ). After production of poly(A)+ mRNA by affinity chromatography (see Chapter 16 ) the RNA can be fractionated once or twice on sucrose gradients to produce a subpopulation of mRNA enriched for a particular species. The RNA fractions from the gradient can be assayed by in vitro translation and subsequent immunoprecipitation or bioassay of the products (see Chapters 19 –Chapters 22 ).
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