丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Penicillan Binding Protein Assay青霉素与细胞膜蛋白结合实验

互联网

959

 

  1. Wash cells with 10 mM Tris pH 8
  2. French press
  3. Slow speed spin
  4. High speed spin
  5. Resuspend in 10 mM Tris
  6. Sonicate 2 x 15 sec to remove ß-lacatamases
  7. Wash in 10 mM Tris
  8. Resuspend in 10 mM Tris
  9. Store at ?70ºC until needed.


Assay:

  1. Incubate membranes with ~5 µCi 3 H-penicillin at 25ºC for 10 minutes
  2. Add 1000X cold penicillin
  3. SDS-PAGE
  4. Incubate in 1 M sodium salicylate for 30 minutes
  5. Dry at 80ºC for 2 hours under vacuum
  6. Expose to photographic film for 3-30 days.

 

ad image
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序