活性氧含量检测试剂盒，用于对实验样本中ROS含量检测，ROS

活性氧含量检测试剂盒（荧光法）

货号：KTB1913-C | 产品分类：核心产品 | 产品中心：细胞分析

产品概述

本产品为Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit，专为活细胞或新鲜组织中活性氧（ROS）含量的精准检测而设计。采用荧光显微技术及流式细胞术兼容方案，适用于氧化应激相关机制研究，如心血管疾病、神经退行性疾病、癌症等领域的细胞代谢分析。

检测原理与适用范围

试剂盒基于DHE（二氢乙啶）荧光探针法，穿透细胞膜后经ROS氧化生成红色荧光产物，荧光强度与ROS水平成正相关。适用于活细胞及新鲜组织样本的定量或半定量分析。

试剂盒组分

• DHE (1000×)
• ROS Positive Control (1000×)

技术优势

• 高特异性荧光探针，适配流式细胞仪与荧光显微镜双平台
• 适用于多种样本类型，提供阳性对照以确保实验可靠性
• 无需复杂预处理，操作流程精简

注意事项

• 建议进行预实验优化检测条件
• 样本需均一化处理（推荐BCA法定量蛋白浓度）
• 需用户自行建立标准曲线进行定量分析
• 操作时需佩戴防护设备（手套、通风橱等），试剂具刺激性/毒性

保存与运输

保存条件：避光-20℃保存，有效期12个月
运输方式：蓝冰冰袋运输

产品规格与价格

背景信息

活性氧（ROS）是氧代谢过程中的天然副产物，参与细胞信号传导与稳态调节。其在氧化应激状态下异常积累，可导致细胞结构损伤，并与心血管疾病、糖尿病、神经退行性疾病及癌症等病理机制密切相关。本试剂盒为研究氧化应激调控细胞内通路提供关键工具。

别名/关键词：活性氧、ROS、氧化应激检测、DHE荧光探针

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit

Catalog Number: KTB1913-C | Product Category: Core Products | Product Center: Cell Analysis

Product Overview

This product is the Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit, specifically designed for the precise detection of reactive oxygen species (ROS) levels in live cells or fresh tissues. It employs fluorescence microscopy and flow cytometry-compatible protocols, making it suitable for research on oxidative stress-related mechanisms, such as cell metabolism analysis in cardiovascular diseases, neurodegenerative disorders, cancer, and other fields.

Detection Principle and Application

The kit utilizes the DHE (Dihydroethidium) fluorescent probe method. After penetrating the cell membrane, DHE is oxidized by ROS to generate a red fluorescent product, with fluorescence intensity positively correlated with ROS levels. It is suitable for quantitative or semi-quantitative analysis of live cell and fresh tissue samples.

Kit Components

• DHE (1000×)
• ROS Positive Control (1000×)

Technical Advantages

• High-specificity fluorescent probe, compatible with both flow cytometry and fluorescence microscopy platforms
• Suitable for various sample types; includes a positive control to ensure experimental reliability
• Simplified operating procedure without complex pretreatment

Precautions

• Pre-experiments are recommended to optimize detection conditions
• Samples require normalization (protein concentration quantification by BCA assay is recommended)
• Users must establish a standard curve for quantitative analysis
• Protective equipment (gloves, fume hood, etc.) must be worn during operation; reagents are irritating/toxic

Storage and Shipping

Storage Conditions: Store protected from light at -20°C; stable for 12 months
Shipping Method: Shipped on blue ice packs

Specifications and Pricing

Background Information

Reactive oxygen species (ROS) are natural byproducts of oxygen metabolism involved in cell signaling and homeostasis regulation. Their abnormal accumulation under oxidative stress conditions can lead to cellular structural damage and is closely associated with pathological mechanisms of cardiovascular diseases, diabetes, neurodegenerative disorders, and cancer. This kit provides a key tool for studying intracellular pathways regulated by oxidative stress.

Aliases/Keywords: Reactive Oxygen Species, ROS, Oxidative Stress Detection, DHE Fluorescent Probe

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult YaKeyin Corporation.