Removal of Counts : NaOH Method

Removal of Counts : NaOH Method

1. Unwrap the membranes and stack them in a pile (Do not dry out the membranes completely).
2. Place them individually into 1L 0.1 N NaOH 0.2 % SDS wash solution for exactly 20 min.
3. Pour off the wash solution and neutralize with 1 L 0.5 M Tris (pH 7.5) 0.2 % SDS for 20 min.
4. Place the membranes on 3 MM paper. Let dry completely between two sheets of 3MM paper and store in plastic bag at 4 C.

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