试剂配方:细菌培养试剂
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1414
LB培养基
NaCl 10 g
Yeast extract 5 g
Peptone 10 g
Add dH 2 O to 1000 ml
Aliquot to 500ml flasks (250 ml per flask). Seal with sealfilm
and autoclave them. Store at room temperature.
LA固体培养基
NaCl 10 g
Yeast extract 5 g
Peptone 10 g
Agar powder 13~15 g
Add dH 2 O to 1000 ml
Aliquot to 500 ml flasks (250 ml per flask). Seal with sealfilm
and autoclave them. Store at room temperature.
X-gal (20mg/ml):
20mg X-gal溶于1ml 二甲基甲酰胺中,-20℃避光保存;
IPTG (200mg/ml):
1g IPTG溶于4ml去离子双蒸水中,定容至5ml,过滤灭菌后-20℃保存;
Amp(100mg/ml):
1g Amp溶于4ml去离子灭菌双蒸水中,定容至5ml,-20℃保存
NaCl 10 g
Yeast extract 5 g
Peptone 10 g
Add dH 2 O to 1000 ml
Aliquot to 500ml flasks (250 ml per flask). Seal with sealfilm
and autoclave them. Store at room temperature.
LA固体培养基
NaCl 10 g
Yeast extract 5 g
Peptone 10 g
Agar powder 13~15 g
Add dH 2 O to 1000 ml
Aliquot to 500 ml flasks (250 ml per flask). Seal with sealfilm
and autoclave them. Store at room temperature.
X-gal (20mg/ml):
20mg X-gal溶于1ml 二甲基甲酰胺中,-20℃避光保存;
IPTG (200mg/ml):
1g IPTG溶于4ml去离子双蒸水中,定容至5ml,过滤灭菌后-20℃保存;
Amp(100mg/ml):
1g Amp溶于4ml去离子灭菌双蒸水中,定容至5ml,-20℃保存