PCR of Gene Rearrangements for the Detection of Minimal Residual Disease in Childhood ALL

IgH FR3

1

5′-ACACGGC(C/T)(G/C)TGTATTACTGT-3′ (2)

3

5′-GTGACCAGGGT(C/T)C C(C/T)TGGCCCCAG-3′ (2)

1.5–30

65–155

75%

8%

4

5′-AACTGCAGAGGAGACGGTGACC-3′ (3)

15–30

80–170

2

5′-GACCAGGGT(C/T)C C(C/T)TGGCCCCAG-3′ ^e

Vδ2-Dδ3

5

5′-CTTGCACCATCAGAGAGAGA-3′ (2)

7

5′-GTTTTTGTACAGGTCTCTGT-3′

10–30

100–150

45%

4%

8

5′-AGGGAAATGCACTTTTGCC-3′ (2)

15–30

110–170

6

5′-TTTTGTACAGGTCTCTGT-3′ ^c

Vδ1-Jδ1

5′-GCCTTACAGCTAGAAGATTC-3′

5′-GTTCCTTTTCCAAAGATGAG-3′

15–30

80–150

5%

25%

VγI-Jγ1/2 ^d

5′-TG(A/C)(C/T)TCTGG(A/G)GTCTATTACTGT-3′

5′-CGATACTTACCTGTGACAAC(C/A)AG-3′

30

80–160

45%

90% ^d

VγII-Jγ-1/2 ^d

5′-AAACAGGACATAGCTACCTACT-3′

5′-CGATACTTACCTGTGACAAACC/AAG-3′

30

80–160

45%

90% ^d

Lead Vγ2-anti Vγ2

5′-GTCATGTCAGCCATTGAGTT-3′

5′-TCTCTCTCTGATGGTGCAAG-3′

15

220

control

control