蛋白质标记

• Biosynthetic labeling (Sefton Lab)
• Biotinylation of Antibody (Contributed by Nanci Donacki)
• 125 I Labeling of Protein using ICl (ScienceXchange)
• Protein (Antibody) Fluorescent Labeling (Mario Roederer)
  Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
  • Fluorescein (FITC)
  • Phycoerythrin (PE)
  • Cy5PE
  • Cy7PE
  • Texas Red (TR)
  • Allophycocyanin (APC)
  • Cy5
  • Cy7APC
  • Cascade Blue
  • Biotin

In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.

• • FITC
  • PE
  • TR-BSA
  • APC
  • Cascade Blue