丁香实验_LOGO
登录
提问
我要登录
|免费注册

DNA制备

丁香实验推荐阅读
DNA克隆

DNA克隆(主要内容如下)・ General Procedure ・ PCR Cloning ・ Subcloning ・ ET Cloning ・ Vector Preparation ・ Ligation Reaction ・ Colony Screening General Pro ...

丁香实验推荐阅读
寡核苷酸的相关操作

In this section you will find techniques related to oligonucleotides such as oligo purification by acrylamide gel annealing two oligos to make double stranded DNA and visualization by UV shadowing an ...

丁香实验推荐阅读
T载体的制作和应用

Also see DNA Cloning§ Making TA Vector (Crawford Lab)T-vectors are linear-blunt-ended plasmids with a few dT's added on by Taq polymerase.§ Making TA Vector (Mounir Izallalen)§ ...

丁香实验推荐阅读
DNA的诱变和甲基化

・ In Vitro Mutagenesis Using Altered Sites (Bowtell Lab)In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab) ・ Site-directed Mutagenesis using PCR (Lazo Lab)PCR site-direc ...

丁香实验推荐阅读
DNA微序列技术

・ Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing Polylysine Slide Preparation "Post-Processing" and ...

丁香实验推荐阅读
RNA点线杂交

RNA点线杂交(Dot and Slot blotting)・ RNA dot blot and slot blot (Beverly Faulkner-Jones)This methods allows the rapid analysis of numerous small samples for the sequence of interest and is less tim ...

丁香实验推荐阅读
差异表达

・ What's Differential Display (GenHunter)Introduction to differential display technique・ Differential Display (Chun-Ming Liu)The following procedures are described:o RNA extract ...

丁香实验推荐阅读
原位转录

Modified Eberwine ("antisense") RNA Amplification Protocol (The MGuide Brown Lab) In Situ RT-PCR (Bo Johansen) ...

丁香实验推荐阅读
体外转录

・ In Vitro RNA Transcription (Promega)For in vitro preparation single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. ・ In Vitro RNA Tra ...

丁香实验推荐阅读
RNA微注射

・ RNA Injection (Hoshi Lab)The oocyte is a useful model for the investigation of the function of usr/localious genes and is a widely used system for the translation of microinjected natural an ...

丁香实验推荐阅读
肽段

・ Designing Your Peptide (Genosys) ・ Handling & Storage of Peptides (Genosys) Two Dimensional Peptide Mapping (Sefton Lab) Synthesizing Peptides (Perkin-Elmer) Solid Phase Peptide ...

丁香实验推荐阅读
细胞核提取

HeLa Cell Nuclei Preparation (John Garland)Prepare nuclear extract from HeLa cell ・ Extract Preparation (Brent Graveley)Preparation of nuclear extract and cytoplasmic extract ・ Pol ...

丁香实验推荐阅读
蛋白质检测

・ Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.・ Protein detection(Silver staining) (ExPASy)T ...

丁香实验推荐阅读
Western 杂交

Western 杂交(主要内容如下)Preparing of Protein Lysates Western Blotting Far Western Blotting Semi Dry Blotting Stripping Membranes Trouble Shooting and Others Preparing Protein LysatesPreparing Protein Lysate ...

丁香实验推荐阅读
蛋白质提取和纯化

蛋白质提取和纯化(主要内容如下)Protein Extraction Protein Purification Protein Precipitation Column Preparatioin Q & A Posted in the Method Forum Protein Extraction Whole Cell Lysate Preparation (UCSF Tumor Immunol ...

丁香实验推荐阅读
蛋白质标记

Biosynthetic labeling (Sefton Lab) Biotinylation of Antibody (Contributed by Nanci Donacki) 125I Labeling of Protein using ICl (ScienceXchange) Protein (Antibody) Fluorescent Labeling (Mario Roederer) ...

丁香实验推荐阅读
蛋白表达

Protein Construct Expression and Purification Procedures(Gimila's Lab) Protein Expression (Mark's Lab) ・ Purification of GST Fused Proteins (Contributed by Chia Jin Ngee)Many people have ...

丁香实验推荐阅读
蛋白质相互作用

Interaction Trap/Trap Two-Hybrid System ・ Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with introduction to ...

丁香实验推荐阅读
蛋白质磷酸化

Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cells or fo ...

丁香实验推荐阅读
转译

・ In Vitro Translation (Promega)Provides general protocol for coupled single-tube tscription/translation reactions for eukaryotic in vitro translation. ・ In vitro Protein Synthesis (P ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序