DNA克隆(主要内容如下)・ General Procedure ・ PCR Cloning ・ Subcloning ・ ET Cloning ・ Vector Preparation ・ Ligation Reaction ・ Colony Screening General Pro ...
In this section you will find techniques related to oligonucleotides such as oligo purification by acrylamide gel annealing two oligos to make double stranded DNA and visualization by UV shadowing an ...
Also see DNA Cloning§ Making TA Vector (Crawford Lab)T-vectors are linear-blunt-ended plasmids with a few dT's added on by Taq polymerase.§ Making TA Vector (Mounir Izallalen)§ ...
・ In Vitro Mutagenesis Using Altered Sites (Bowtell Lab)In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab) ・ Site-directed Mutagenesis using PCR (Lazo Lab)PCR site-direc ...
・ Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing Polylysine Slide Preparation "Post-Processing" and ...
RNA点线杂交(Dot and Slot blotting)・ RNA dot blot and slot blot (Beverly Faulkner-Jones)This methods allows the rapid analysis of numerous small samples for the sequence of interest and is less tim ...
・ What's Differential Display (GenHunter)Introduction to differential display technique・ Differential Display (Chun-Ming Liu)The following procedures are described:o RNA extract ...
Modified Eberwine ("antisense") RNA Amplification Protocol (The MGuide Brown Lab) In Situ RT-PCR (Bo Johansen) ...
・ In Vitro RNA Transcription (Promega)For in vitro preparation single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. ・ In Vitro RNA Tra ...
・ RNA Injection (Hoshi Lab)The oocyte is a useful model for the investigation of the function of usr/localious genes and is a widely used system for the translation of microinjected natural an ...
・ Designing Your Peptide (Genosys) ・ Handling & Storage of Peptides (Genosys) Two Dimensional Peptide Mapping (Sefton Lab) Synthesizing Peptides (Perkin-Elmer) Solid Phase Peptide ...
HeLa Cell Nuclei Preparation (John Garland)Prepare nuclear extract from HeLa cell ・ Extract Preparation (Brent Graveley)Preparation of nuclear extract and cytoplasmic extract ・ Pol ...
・ Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.・ Protein detection(Silver staining) (ExPASy)T ...
Western 杂交(主要内容如下)Preparing of Protein Lysates Western Blotting Far Western Blotting Semi Dry Blotting Stripping Membranes Trouble Shooting and Others Preparing Protein LysatesPreparing Protein Lysate ...
蛋白质提取和纯化(主要内容如下)Protein Extraction Protein Purification Protein Precipitation Column Preparatioin Q & A Posted in the Method Forum Protein Extraction Whole Cell Lysate Preparation (UCSF Tumor Immunol ...
Biosynthetic labeling (Sefton Lab) Biotinylation of Antibody (Contributed by Nanci Donacki) 125I Labeling of Protein using ICl (ScienceXchange) Protein (Antibody) Fluorescent Labeling (Mario Roederer) ...
Protein Construct Expression and Purification Procedures(Gimila's Lab) Protein Expression (Mark's Lab) ・ Purification of GST Fused Proteins (Contributed by Chia Jin Ngee)Many people have ...
Interaction Trap/Trap Two-Hybrid System ・ Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with introduction to ...
Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cells or fo ...
・ In Vitro Translation (Promega)Provides general protocol for coupled single-tube tscription/translation reactions for eukaryotic in vitro translation. ・ In vitro Protein Synthesis (P ...