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        DNA微序列技术

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        1264

         

                 Protocols for Making Drosophila Arrays (Stanford U.)
        Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,  Polylysine Slide Preparation, "Post-Processing" and Direct Labeling of cDNA probes.
          

                 Preparation of Slides (Brown Lab)
          

                 Preparation of DNA Samples (Brown Lab)
          

                 Post-Processing of Arrays (Brown Lab)
          

                 Preparation of Fluorescent DNA Probe from Yeast mRNA  (Brown Lab)
          

                 Preparation of Fluorescent Probe from Human RNA (Brown Lab)
          

                 Preparation of Fluorescent Probe from E. coli RNA (Brown Lab)
          

                 Preparation of Fluorescent DNA Probe from Genomic DNA (Brown Lab)
          

                 Cyanine Dye HPLC Purification (Brown Lab)
          

                 Modified Eberwine ("antisense") RNA Amplification Protocol   (Brown Lab)
          

                 Hybridization of Arrays (Brown Lab)
          

                 Preparation of Total RNA from Cultured Human Cells (Telechem)
          

                 Preparation of PolyA+ mRNA from Total Human RNA (Telechem)
          

                 Amplification and Purification of cDNAs for Microarray Manufacture (Telechem)
         

                 Microarray Manufacture and Processing (Telechem)
          

                 Generating Control mRNAs by In Vitro Transcription (Telechem)
          

                 Generating Fluorescent cDNA Controls by Linear PCR (Telechem)
          

                 Preparation of Fluorescent Probes from Total Human mRNA (Telechem)
         

                 cDNA Microarray Hybridization and Washing (Telechem)
         

                 Gene Expression Analysis with Microarrays (Telechem)
         

                 Mutation Detection with Oligonucleotide Microarrays (Telechem)
          

                 Comparative Gene Expression Study Using Microarray (Jeremy Buhler) 
           

                 Microarray Hybridization Protocols (Childs / Kucherlapati Lab)

         

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