数据库

实验试剂 Column equilibration buffer:1xPBS/0.5M NaClHigh salt wash buffer:50mM Tris 8.0/2M NaClElution buffer:200mM Glycine/150mM NaCl PH2.2Neutralization buffer:2M Tris PH8.0Bacteria breakage buffer ...

实验试剂 1. STE 0.1mol/L NaCl 10mmol/L Tris.Cl(pH8.0) 1mmol/L EDTA(pH8.0) 2. 溶液Ｉ 50mmol/L葡萄糖 25mmol/L Tris.Cl(pH8.0) 10mmol/L EDTA(pH8.0) 溶液Ｉ可成批配制，在6.895x104Pa高压下蒸气灭菌15分钟，贮存于４℃。 3. 溶菌酶溶液10mg/ml，溶于10mmol ...

实验试剂 Regents to be supplied by user1. Proteinase K (20mg/ml)2. Isopropanol 3. 70% ethanol 实验设备 Equipments to be supplied by user1. Microcentrifuge capable of 14000 x g2. Nuclease-free 1.5 ml mic ...

实验原理 E.Z.N.A.® SQ Tissue RNA uses a highly efficient solution based system to provide a convenient fast reliable and non-toxic method to isolate high quality RNA from various samples. Samples are fir ...

实验步骤 1.Preparation of Running Gel Solution1) Add to a 50 ml cylinder:DD-H2O 26 ml30% acrylamide stock (37.5:1 Acryl/Bis) 10.5 ml2M Tris 8.8 8.4 ml20% SDS 0.23 ml2) Cover with a piece of Parafilm and ...

实验原理 A large selection of BLOCK-iT™ RNAi products is available from Invitrogen to facilitate RNAi analysis in mammalian and invertebrate systems. The BLOCK-iT™ Inducible H1 RNAi Entry Vector Kit supp ...

实验原理 酶是指化学本质为蛋白质的生物催化剂。在一定条件下，酶促化学反应进行的能力即称为酶活性(酶活力)。影响酶活性的因素是多方面的，如温度、PH及某些化学物质等都会影响酶的催化活性。在一定条件下，能使酶活性达到最高时的温度即酶的最适温度，而能使酶活性达到最高时的PH即酶的最适PH。例如，唾液淀粉酶的最适温度是37℃，而其最适PH是6.8。能增高酶活性的物质称为酶的激活剂，能降低酶活性却又不使酶 ...

实验试剂 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc Clifton NJ) 6L 0.1M HCl6L 0.1 M NaOH2L 0.1 M MgSO42L 10x Column Buffer~10 L 1x column buffer 10 M NaOH300 ml Homogenization bu ...

实验步骤 1. 动物模型 选用80只250g±20成年雄性Wistar大鼠，随机等量地分为实验组和对照组（正常力）。实验组拔除左侧上颌（B区）所有磨牙。这样，实验组动物的D区磨牙因丧失对颌牙导致力丧失，而C区磨牙功能代偿性增强则构成力增强的动物模型。两组动物分别在实验后6小时、1天、2天、3天、1周、2周、3周和4周各处死5只，共16组。 2. 组织标本制备 实验动物采用4％多聚甲醛心内灌注的方 ...

实验试剂 1. Recombinant kinase (II) 2. PATP analog (I II) 3. Sepharose CL-6B beads (I) 4. Lipofectamine (I) 5. Agonist (I) e.g. epidermal growth factor serum platelet-derived growth factor 6. FLAG ...

实验试剂 1. 100% ethanol2.75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50 mg tissue 1 - 3x107 cells 0.1 ml liquid sample2. Centrifugatio ...

实验步骤 为了检测小胶质细胞的吞噬功能，利用凋亡的神经祖细胞作为目标，因为在体外环境下它能最模拟自然条件下小胶质细胞的吞噬目标。1. 将神经祖细胞（NPC）置于0.1M PBS 2mg/ml木瓜蛋白酶（Worthington）溶液中进行分离，37ºC作用30min。2. 将分离的NPC 在紫外光下处理15-20min。注：此步应该在一个很薄的塑料培养皿中进行，厚的塑料（如15或50毫升锥形管）将 ...

实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube2. Water bath incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes 实验步骤 1 ...

实验原理 E.Z.N.A.® SQ Tissue RNA uses a highly efficient solution based system to provide a convenient fast reliable and non-toxic method to isolate high quality RNA from various samples. Samples are fir ...

实验试剂 1. Complete growth medium pre-warmed to 37℃2. Balanced salt solution such as Dulbecco’s Phosphate Buffered Saline (DPBS) containing no calcium magnesium or phenol red3. Dissociation reagent such ...

实验步骤 1. Measure the volume of sample and adjust the sample volume to 100ul with DEPC-Water and proceed to step 2.2. Add 350ul QVL Lysis buffer and mix by vortexing at maximum speed for 15 seconds. Wh ...

实验原理 1. 单向琼脂扩散试验是一种定量试验，将抗体混合于琼脂内，倾注于玻片或平皿上，凝固后在琼脂上打孔，再将抗原标本加入孔内，经过一定的时间，在孔的周围出现抗原抗体复合物形成的沉淀环，环的大小与抗原含量和扩散时间相关。用不同浓度的抗原制成标准曲线，则未知标本中的抗原含量即可从准标曲线中求出。2. 抗原和抗体加到琼脂板上相对应的孔中，两者各自向四周扩散，如两者相对应，浓度比例合适，则经一定时间 ...

实验材料 NameCompanyCatalog NumberCommentsSodium chlorideSigmaS7653PBSPotassium chlorideSigmaP9333PBSSodium phosphate dibasicSigmaS7907PBSPotassium phosphate monobasicSigmaP5655PBSGlucoseSigmaG5400Isolat ...

实验步骤 The following protocol used formalin-fixed paraffin-embeddedhuman tonsil tissue (supplied by the Alfred Hospital Australia).1.Deparaffinize and rehydrate the tissue section. 2.Perform heat-ind ...

实验试剂 1. 细胞裂解液：10 mmol／L Tris-HCL(pH 8.0)，150mmol／LNaCl，10mmol／LEDTA，0.4％SDS，蛋白酶K 100ug／m1。2. RNase(10 mg／m1)：RNase溶解于100 mmol／LTris-HCl(pH7.5)及15 mmol／L NaCl中。煮沸15 min后缓慢冷却至室温，-20℃储存。3. TE缓冲液：Tris-HC ...