SQ Blood Mini Protocol for 50ul Clotted Blood
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实验试剂
Regents to be supplied by user
实验设备
Equipments to be supplied by user
1. Microcentrifuge capable of 14,000 x g
2. Nuclease-free 1.5 ml microcentrifuge tubes
实验步骤
1. Transfer the 50ul blood include any liquid residual into a 1.5 ml centrifuge tube.
2. Add 550ul WTL Buffer and pipet up an down a few times to mix.
3. Add 3 ul Proteinase K solution (20mg/ml) and mix by inverting 20 times.
4. Incubate at 55°C for 1 hour to overnight until clots has dissolved.
5. Place the tube on ice for 1 minute.
13. Invert the tube on a clean adsorbent paper towel and air dry the pellet for 10-15 minutes.
14. Add 20 ul of DNA rehydration solution (Buffer EB) and vortex for 1 minute to mix.
16. Store DNA at 2-8°C. For long-term storage, store at -20°C.
